Journal article
Arrestin-Domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles
S Anand, N Foot, CS Ang, KM Gembus, S Keerthikumar, CG Adda, S Mathivanan, S Kumar
Proteomics | WILEY | Published : 2018
Abstract
Extracellular vesicles (EVs) are lipid-bilayered vesicles that are released by multiple cell types and contain nucleic acids and proteins. Very little is known about how the cargo is packaged into EVs. Ubiquitination of proteins is a key posttranslational modification that regulates protein stability and trafficking to subcellular compartments including EVs. Recently, arrestin-domain containing protein 1 (Arrdc1), an adaptor for the Nedd4 family of ubiquitin ligases, has been implicated in the release of ectosomes, a subtype of EV that buds from the plasma membrane. However, it is currently unknown whether Arrdc1 can regulate the release of exosomes, a class of EVs that are derived endocytic..
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Awarded by Australian Research Council
Funding Acknowledgements
S.A. and N.F. contributed equally to this work. S.M. is supported by Australian Research Council DECRA (DE150101777) and S.K. by a National Health and Medical Research Council (NHMRC) Senior Principal Research Fellowship (1103006). The project is funded by NHMRC project grant (1122437). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This research was supported by use of the NeCTAR Research Cloud, a collaborative Australian research platform supported by the National Collaborative Research Infrastructure Strategy.