Journal article

Influenza A(H5N1) viruses with A(H9N2) single gene (matrix or PB1) reassortment isolated from Cambodian live bird markets

Annika Suttie, Erik A Karlsson, Yi-Mo Deng, Srey Viseth Horm, Sokhoun Yann, Songha Tok, San Sorn, Davun Holl, Sothyra Tum, Aeron C Hurt, Andrew R Greenhill, Ian G Barr, Paul F Horwood, Philippe Dussart



Live bird market surveillance for avian influenza viruses in Cambodia in 2015 has led to the detection of two 7:1 reassortant influenza A(H5N1) clade viruses. These reassortant strains, designated A/duck/Cambodia/Z564W35M1/2015 and A/chicken/Cambodia/Z850W49M1/2015, both contained a single gene (PB1 and matrix gene, respectively) from concurrently circulating A(H9N2) influenza viruses. All other viral genes from both isolates clustered with A(H5N1) clade viruses. Continued and prolonged co-circulation of influenza A(H5N1) and A(H9N2) viruses in Cambodian live bird markets may present a risk for the emergence of novel influenza reassortant viruses with negative agricultural a..

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Awarded by Office of the Assistant Secretary for Preparedness and Response in the U.S. Department of Health and Human Services (HHS)

Awarded by US Agency for International Development

Funding Acknowledgements

The authors thank the field and laboratory teams at the Institute Pasteur in Cambodia and the National Animal Health and Production Research Institute for their efforts. We also thank the vendors from the participating live bird markets for their cooperation with our study team. This publication is the result of work conducted under a cooperative agreement with the Office of the Assistant Secretary for Preparedness and Response in the U.S. Department of Health and Human Services (HHS), grant number IDSEP140020-01-00. Its contents and conclusions are solely the responsibility of the authors and do not represent the official views of HHS. The study was also funded, in part, by the US Agency for International Development (grant No. AID-442-G-14-00005). Annika Suttie is funded by an Australian Government Research Training Program Scholarship and a Faculty of Science and Technology Research Scholarship from Federation University. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health.