Journal article
Diet during Pregnancy is Implicated in the Regulation of Hypothalamic RNA Methylation and Risk of Obesity in Offspring
A Kaspi, I Khurana, M Ziemann, T Connor, B Spolding, P Zimmet, K Walder, A El-Osta
Molecular Nutrition and Food Research | WILEY | Published : 2018
Abstract
Scope: Early life nutrition has long-lasting influence in adults through key mediators that modulate epigenetic states, although the determinants involved that underlie this response remain controversial. Because of the similarities between metabolic, physiological, and endocrine changes and those occurring in human type 2 diabetes, we studied the interaction of diet during pregnancy regulating RNA adenosine methylation (N6-methyladenosine [m6A]) and the transcriptome in Psammomys obesus. Methods and results: Breeding pairs were randomly allocated standard diet (total digestible energy 18 MJ kg−1) or low-fat diet (15 MJ kg−1). Offspring were weaned onto the low-fat diet at 4 weeks of age and..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
A.K. and I.K. contributed equally to this work. I.K. T.C., and B.S. performed the investigation. I.K. and A.K. performed the validation experiments and data visualization. M.Z., A.K., and I.K. were involved in data curation, soft-ware, and methodology. M.Z., A.K., and I.K. were involved in provision of resources and methodology. K.W., P.Z., and A.E.-O. were involved in conceptualization, supervision, project administration, and funding acquisition. A. E.-O. was involved in original draft. All the authors were involved in reviewing and approving the manuscript. The authors thank Ross Lazarus (Baker IDI Heart and Diabetes Institute) for bioinformatics support in the preparation of this manuscript. The authors acknowledge fellowship for A.E. (APP0526681), support from the National Health and Medical Research Council (NHMRC) and the National Natural Science Foundation of China. The project would not have been possible without the kind support from the Moshe Meydan Donation.