EPO does not promote interaction between the erythropoietin and beta-common receptors

KS Cheung Tung Shing; SE Broughton; TL Nero; K Gillinder; MD Ilsley; H Ramshaw; AF Lopez; MDW Griffin; MW Parker; AC Perkins; U Dhagat

Journal article

EPO does not promote interaction between the erythropoietin and beta-common receptors

KS Cheung Tung Shing, SE Broughton, TL Nero, K Gillinder, MD Ilsley, H Ramshaw, AF Lopez, MDW Griffin, MW Parker, AC Perkins, U Dhagat

Scientific Reports | NATURE PORTFOLIO | Published : 2018

DOI: 10.1038/s41598-018-29865-x

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Abstract

A direct interaction between the erythropoietin (EPOR) and the beta-common (βc) receptors to form an Innate Repair Receptor (IRR) is controversial. On one hand, studies have shown a functional link between EPOR and βc receptor in tissue protection while others have shown no involvement of the βc receptor in tissue repair. To date there is no biophysical evidence to confirm a direct association of the two receptors either in vitro or in vivo. We investigated the existence of an interaction between the extracellular regions of EPOR and the βc receptor in silico and in vitro (either in the presence or absence of EPO or EPO-derived peptide ARA290). Although a possible interaction between EPOR an..

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University of Melbourne Researchers

Tracy Nero Author

Michael Griffin Author

Michael Parker Author

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Funding Acknowledgements

We thank Dr Timothy R Hercus, Centre for Cancer Biology, for providing the beta c construct. We also thank Dr. Yee-Foong Mok for his help with the AUC experiments and Larissa Doughty for guidance with the SPR assays. We thank the National Health and Medical Research Council of Australia (NHMRC) (Grant number APP1071897), Cancer Council of Victoria (Grant number APP1122401) and the Australian Cancer Research Foundation for supporting this work. Funding from the Victorian Government Operational Infrastructure Support Scheme to St Vincent's Institute is acknowledged. K.S.C.T.S is a PhD student recipient of scholarships from the Department of Biochemistry and Molecular Biology, University of Melbourne and from St Vincent's Institute of Medical Research. M.D.W.G is the recipient of an Australian Research Council Future Fellowship (project number FT140100544). M.W.P. is an NHMRC Research Fellow (APP1117183).