High-throughput screening for small molecule inhibitors of the type-I interferon signaling pathway
Elita Yuliantie, Xinchuan Dai, Dehua Yang, Peter J Crack, Ming-Wei Wang
Acta Pharmaceutica Sinica B | INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES | Published : 2018
Interferons (IFNs) are cytokines with fundamental roles in resistance to infections, cancer and other diseases. Type-I IFNs, interferon α (IFN-α) and interferon β (IFN-β), act through a shared receptor complex (IFNAR) comprised of IFNAR1 and IFNAR2 subunits. Binding of type-I IFN to IFNAR1 will robustly activate Janus activated kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway. Aberrant activation of the type-I IFN response results in a spectrum of disorders called interferonopathies. The purpose of this research is to develop an assay for high-throughput screening (HTS) of small molecule inhibitors of the type-I IFN signaling pathway. Inhibition of type-I ..View full abstract
Awarded by Shanghai Science and Technology Development Fund
Awarded by Thousand Talents Program in China
We are indebted to Caihong Zhou, Ji Wu and Qiang Shen for technical assistance. This work was partially supported by grants from Shanghai Science and Technology Development Fund (to MWW: 15DZ2291600) and the Thousand Talents Program in China (to MWW: 166). PJC was a recipient of an Australian Research Council Future Fellowship and CAS President's International Fellowship Initiative (PIFI). The funders had no role in study design, data collection and analysis, decision to publish, or manuscript preparation.