Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome

G Keeble-Gagnère; P Rigault; J Tibbits; R Pasam; M Hayden; K Forrest; Z Frenkel; A Korol; BE Huang; C Cavanagh; J Taylor; M Abrouk; A Sharpe; D Konkin; P Sourdille; B Darrier; F Choulet; A Bernard; S Rochfort; A Dimech; N Watson-Haigh; U Baumann; P Eckermann; D Fleury; A Juhasz; S Boisvert; MA Nolin; J Doležel; H Šimková; H Toegelová; J Šafář; MC Luo; F Câmara; M Pfeifer; D Isdale; J Nyström-Persson; DH Koo; M Tinning; D Cui; Z Ru; R Appels

Journal article

Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome

G Keeble-Gagnère, P Rigault, J Tibbits, R Pasam, M Hayden, K Forrest, Z Frenkel, A Korol, BE Huang, C Cavanagh, J Taylor, M Abrouk, A Sharpe, D Konkin, P Sourdille, B Darrier, F Choulet, A Bernard, S Rochfort, A Dimech Show all

Genome Biology | Published : 2018

DOI: 10.1186/s13059-018-1475-4

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Abstract

Background: Numerous scaffold-level sequences for wheat are now being released and, in this context, we report on a strategy for improving the overall assembly to a level comparable to that of the human genome. Results: Using chromosome 7A of wheat as a model, sequence-finished megabase-scale sections of this chromosome were established by combining a new independent assembly using a bacterial artificial chromosome (BAC)-based physical map, BAC pool paired-end sequencing, chromosome-arm-specific mate-pair sequencing and Bionano optical mapping with the International Wheat Genome Sequencing Consortium RefSeq v1.0 sequence and its underlying raw data. The combined assembly results in 18 super-..

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