Journal article

Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome.

Gabriel Keeble-Gagnère, Philippe Rigault, Josquin Tibbits, Raj Pasam, Matthew Hayden, Kerrie Forrest, Zeev Frenkel, Abraham Korol, B Emma Huang, Colin Cavanagh, Jen Taylor, Michael Abrouk, Andrew Sharpe, David Konkin, Pierre Sourdille, Benoît Darrier, Frédéric Choulet, Aurélien Bernard, Simone Rochfort, Adam Dimech Show all

Genome Biol | Published : 2018

Abstract

BACKGROUND: Numerous scaffold-level sequences for wheat are now being released and, in this context, we report on a strategy for improving the overall assembly to a level comparable to that of the human genome. RESULTS: Using chromosome 7A of wheat as a model, sequence-finished megabase-scale sections of this chromosome were established by combining a new independent assembly using a bacterial artificial chromosome (BAC)-based physical map, BAC pool paired-end sequencing, chromosome-arm-specific mate-pair sequencing and Bionano optical mapping with the International Wheat Genome Sequencing Consortium RefSeq v1.0 sequence and its underlying raw data. The combined assembly results in 18 super-..

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University of Melbourne Researchers

Grants

Awarded by Grains Research and Development Corporation


Awarded by Department of Industry, Innovation, Science, Research and Tertiary Education, Australian Government


Awarded by Commonwealth Scientific and Industrial Research Organisation


Awarded by BioPlatform Australia (BPA)


Awarded by Victorian Department of Economic Development, Jobs, Transport and Resources


Awarded by National Science Foundation


Awarded by INB ("Instituto National de Bioinformatica")


Awarded by Czech Ministry of Education Youth and Sports