Redox Modulating Factors Affect Longevity Regulation in Rotifers
Lilla Macsai, Zita Olah, Ashley I Bush, Bence Galik, Rita Onody, Janos Kalman, Zsolt Datki
The Journals of Gerontology: Series A | OXFORD UNIV PRESS INC | Published : 2019
Rotifers are microinvertebrate models to study the phylogenetically based mechanisms of aging. Our study aimed to develop a physiological system with electron deprivation via a chemical electron carrier/acceptor pair together with extreme caloric restriction (ECR). Middle-aged Philodina acuticornis rotifers were treated with combinations of phenazine methosulfate (PMS, electron carrier) and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT, electron acceptor) for a period of 72 hours under total food deprivation (preselection). The ability of XTT to be reduced was confirmed both in vitro (with NADH) and in vivo (with live rotifers). Subsequently, the res..View full abstract
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. This work was supported by the Australian National Health and Medical Research Council to A.B.