DNA Methylation Profiling of Breast Cancer Cell Lines along the Epithelial Mesenchymal Spectrum-Implications for the Choice of Circulating Tumour DNA Methylation Markers
Viet-Phuong Le Anh, Marcin Szaumkessel, Tuan Zea Tan, Jean-Paul Thiery, Erik W Thompson, Alexander Dobrovic
International Journal of Molecular Sciences | MDPI | Published : 2018
(1) Background: Epithelial⁻mesenchymal plasticity (EMP) is a dynamic process whereby epithelial carcinoma cells reversibly acquire morphological and invasive characteristics typical of mesenchymal cells. Identifying the methylation differences between epithelial and mesenchymal states may assist in the identification of optimal DNA methylation biomarkers for the blood-based monitoring of cancer. (2) Methods: Methylation-sensitive high-resolution melting (MS-HRM) was used to examine the promoter methylation status of a panel of established and novel markers in a range of breast cancer cell lines spanning the epithelial⁻mesenchymal spectrum. Pyrosequencing was used to validate the MS-HRM resul..View full abstract
Awarded by National Collaborative Research Program of the National Breast Cancer Foundation (NBCF; Australia)
Awarded by National Health and Medical Research Council of Australia
Awarded by NBCF
This work was supported by a National Collaborative Research Program of the National Breast Cancer Foundation (NBCF; Australia) to E.W.T. and A.D. (CG-10-04), a National Health and Medical Research Council of Australia grant to E.W.T. and A.D. (1027527), and grants to A.D. by the NBCF (CG-08-07 and CG-12-07), the Cancer Council of Victoria, and the Victorian Cancer Agency. A.V.-P.L. was supported by an international research scholarship from the University of Melbourne and the Cancer Therapeutics CRC (CTx) PhD top-up scholarship. The Olivia Newton-John Cancer Research Institute is supported by the Victorian Government Operational and Infrastructure Support Grant. The Translational Research Institute is supported by a grant from the Australian Government.