Epstein-Barr virus protein EB2 stimulates cytoplasmic mRNA accumulation by counteracting the deleterious effects of SRp20 on viral mRNAs
Franceline Juillard, Quentin Bazot, Fabrice Mure, Lionel Tafforeau, Christophe Macri, Chantal Rabourdin-Combe, Vincent Lotteau, Evelyne Manet, Henri Gruffat
NUCLEIC ACIDS RESEARCH | OXFORD UNIV PRESS | Published : 2012
The Epstein-Barr Virus (EBV) protein EB2 (also called Mta, SM and BMLF1), is an essential nuclear protein produced during the replicative cycle of EBV. EB2 is required for the efficient cytoplasmic accumulation of viral mRNAs derived from intronless genes. EB2 is an RNA-binding protein whose expression has been shown to influence RNA stability, splicing, nuclear export and translation. Using a yeast two-hybrid screen, we have identified three SR proteins, SF2/ASF, 9G8 and SRp20, as cellular partners of EB2. Then, by using siRNA to deplete cells of specific SR proteins, we found that SRp20 plays an essential role in the processing of several model mRNAs: the Renilla luciferase reporter mRNA, ..View full abstract
Awarded by Agence Nationale pour la Recherche
Institut National de la Sante et de la Recherche Medicale (INSERM); Agence Nationale pour la Recherche [RPV06120CSA and RPV09056CSA to E. M.]; Pole de Competitivite Lyon-Biopole; and Cluster de Recherche Rhone-Alpes en Infectiologie. Q. B. is recipient of a fellowship from the Ministere de la Recherche et de la Technologie (MRT) and of Association pour la Recherche sur le Cancer. F.J. is the recipient of a fellowship from the Ministere de la Recherche et de la Technologie (MRT) and the Fondation pour la Recherche Medicale (FRM); E. M. is a CNRS scientist. Funding for open access charge: INSERM.