Membrane vesicles from Pseudomonas aeruginosa activate the noncanonical inflammasome through caspase-5 in human monocytes

Grants

Funding Acknowledgements

Pseudomonas aeruginosa PA103 Delta pilA was kindly provided by Cynthia B Whitchurch (ithree institute, University of Technology Sydney, Sydney, Australia). The WT, Nlrp3<SUP>-/-</SUP>, Nlrc4<SUP>-/-</SUP>, Aim2<SUP>-/-</SUP>, Mal<SUP>-/-</SUP>, Myd88-Trif<SUP>-/-</SUP>, Tlr2<SUP>-/-</SUP> and ASC-cerulean immortalized macrophage cell lines were generously donated by Douglas Golenbock (University of Massachusetts Medical School, Boston, USA) and Eike Latz (University of Bonn, Bonn, Germany). Bone marrow from Tlr4<SUP>-/-</SUP> mice was kindly provided by Professor Matt Sweet and Mr James Curson (Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia). The authors acknowledge the facilities, scientific and technical assistance of Monash Micro Imaging, Monash University, Victoria, Australia. This project was supported by funding from the Australian Research Council to RLF and KJS (Discovery grant DP120104911) and the National Health and Medical Research Council (NHMRC) to RLF (Project grant APP1030243). Research at the Hudson Institute of Medical Research is supported by the Victorian Government's Operational Infrastructure Support Program. RLF and KJS are supported by NHMRC Senior Research Fellowships (APP1079904 and APP1059729, respectively). RC and NJB were funded by the NHMRC and an Australian Postgraduate Award, respectively.