Journal article

The Three Members of the Arabidopsis Glycosyltransferase Family 92 are Functional beta-1,4-Galactan Synthases

Berit Ebert, Devon Birdseye, April JM Liwanag, Tomas Laursen, Emilie A Rennie, Xiaoyuan Guo, Michela Catena, Carsten Rautengarten, Solomon H Stonebloom, Pawel Gluza, Venkataramana R Pidatala, Mathias CF Andersen, Roshan Cheetamun, Jenny C Mortimer, Joshua L Heazlewood, Antony Bacic, Mads H Clausen, William GT Willats, Henrik V Scheller

PLANT AND CELL PHYSIOLOGY | OXFORD UNIV PRESS | Published : 2018

Abstract

Pectin is a major component of primary cell walls and performs a plethora of functions crucial for plant growth, development and plant-defense responses. Despite the importance of pectic polysaccharides their biosynthesis is poorly understood. Several genes have been implicated in pectin biosynthesis by mutant analysis, but biochemical activity has been shown for very few. We used reverse genetics and biochemical analysis to study members of Glycosyltransferase Family 92 (GT92) in Arabidopsis thaliana. Biochemical analysis gave detailed insight into the properties of GALS1 (Galactan synthase 1) and showed galactan synthase activity of GALS2 and GALS3. All proteins are responsible for adding ..

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Grants

Awarded by US Department of Energy, Office of Science, Office of Biological and Environmental Research


Awarded by Danish Strategic Research Council


Awarded by Australian Research Council Future Fellowships


Awarded by Villum Foundation


Awarded by ARC Centre of Excellence in Plant Cell Walls


Funding Acknowledgements

This work was supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy, the Danish Strategic Research Council [Set4Future 11-116795] to H.V.S. and M.H.C., Australian Research Council Future Fellowships [FT160100276, FT130101165] to B.E. and J.L.H., the Villum Foundation (PLANET project) and the Novo Nordisk Foundation (Biotechnology-based Synthesis and Production Research) to M.C.F.A., M.H.C., the Villum Foundation (95-300-73023) to T.L., and the ARC Centre of Excellence in Plant Cell Walls [CE110001007] to A.B.