Journal article

Beyond Global Charge: Role of Amine Bulkiness and Protein Fingerprint on Nanoparticle-Cell Interaction

David Burnand, Ana Milosevic, Sandor Balog, Miguel Spuch-Calvar, Barbara Rothen-Rutishauser, Jorn Dengjel, Calum Kinnear, Thomas L Moore, Alke Petri-Fink

SMALL | WILEY-V C H VERLAG GMBH | Published : 2018

Abstract

Amino groups presented on the surface of nanoparticles are well-known to be a predominant factor in the formation of the protein corona and subsequent cellular uptake. However, the molecular mechanism underpinning this relationship is poorly defined. This study investigates how amine type and density affect the protein corona and cellular association of gold nanoparticles with cells in vitro. Four specific poly(vinyl alcohol-co-N-vinylamine) copolymers are synthesized containing primary, secondary, or tertiary amines. Particle cellular association (i.e., cellular uptake and surface adsorption), as well as protein corona composition, are then investigated. It is found that the protein corona ..

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University of Melbourne Researchers

Grants

Awarded by Swiss National Science Foundation through an Early Postdoc.Mobility


Awarded by ARC


Funding Acknowledgements

This project was supported by the Chemistry Department at the Universite de Fribourg, the Adolphe Merkle Foundation, and the Swiss National Science Foundation through the National Center of Competence in Research Bio-Inspired Materials. C.K. acknowledges support from the Swiss National Science Foundation through an Early Postdoc.Mobility (P2FRP2_158730) and the ARC for support through CE170100026.