Journal article

Integrative proteomics and bioinformatic prediction enable a high-confidence apicoplast proteome in malaria parasites

Michael J Boucher, Sreejoyee Ghosh, Lichao Zhang, Avantika Lal, Se Won Jang, An Ju, Shuying Zhang, Xinzi Wang, Stuart A Ralph, James Zou, Joshua E Elias, Ellen Yeh

PLOS BIOLOGY | PUBLIC LIBRARY SCIENCE | Published : 2018

Abstract

Malaria parasites (Plasmodium spp.) and related apicomplexan pathogens contain a nonphotosynthetic plastid called the apicoplast. Derived from an unusual secondary eukaryote-eukaryote endosymbiosis, the apicoplast is a fascinating organelle whose function and biogenesis rely on a complex amalgamation of bacterial and algal pathways. Because these pathways are distinct from the human host, the apicoplast is an excellent source of novel antimalarial targets. Despite its biomedical importance and evolutionary significance, the absence of a reliable apicoplast proteome has limited most studies to the handful of pathways identified by homology to bacteria or primary chloroplasts, precluding our a..

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University of Melbourne Researchers

Grants

Awarded by National Institutes of Health


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

National Institutes of Health https://www.nih.gov/ (grant number K08 AI097239). Awarded to E.Y. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. National Institutes of Health https://www.nih.gov/ (grant number DP5 OD012119). Awarded to E.Y. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Burroughs Wellcome Fund https://www.bwfund.org/. Career Award for Medical Scientists awarded to E.Y. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Chan Zuckerberg Biohub https://www.czbiohub.org/. E.Y. and J.Z. are Chan Zuckerberg Biohub Investigators. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Stanford Bio-X https://biox.stanford.edu/. Interdisciplinary Initiatives Seed Grant awarded to E.Y. and J.E.E. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. National Science Foundation https://www.nsf.gov/. CRII grant awarded to J.Z. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. National Health and Medical Research Council https://www.nhmrc.gov.au/. RD Wright Biomedical fellowship awarded to S.A.R. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Stanford Graduate Fellowship https://vpge.stanford.edu/fellowships-funding/sgf. William R. and Sara Hart Kimball fellowship awarded to M. J.B. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.