Journal article
Elucidating the Influences of Size, Surface Chemistry, and Dynamic Flow on Cellular Association of Nanoparticles Made by Polymerization-Induced Self-Assembly
SY Khor, MN Vu, EH Pilkington, APR Johnston, MR Whittaker, JF Quinn, NP Truong, TP Davis
Small | WILEY-V C H VERLAG GMBH | Published : 2018
Abstract
The size and surface chemistry of nanoparticles dictate their interactions with biological systems. However, it remains unclear how these key physicochemical properties affect the cellular association of nanoparticles under dynamic flow conditions encountered in human vascular networks. Here, the facile synthesis of novel fluorescent nanoparticles with tunable sizes and surface chemistries and their association with primary human umbilical vein endothelial cells (HUVECs) is reported. First, a one-pot polymerization-induced self-assembly (PISA) methodology is developed to covalently incorporate a commercially available fluorescent dye into the nanoparticle core and tune nanoparticle size and ..
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Awarded by Australian Research Council
Funding Acknowledgements
S.Y.K. and M.N.V. contributed equally to this work. Electron microscopy was performed at the Bio21 Advanced Microscopy Facility, The University of Melbourne. This work was carried out within the Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology (Project No. CE140100036). The authors would like to thank Mr. Cameron Nowell for his technical support on confocal microscopy. S.Y.K. and E.H.P. acknowledge the financial support from the Australian Government Research Training Program Scholarship. M.N.V. acknowledges the financial support of Vietnamese Government and the Faculty of Pharmacy and Pharmaceutical Sciences, Monash University. N.P.T. acknowledges the award of a DECRA Fellowship from the ARC (DE180100076). J.F.Q. acknowledges receipt of a Future Fellowship from the ARC (FT170100144). T.P.D. is grateful for the award of an Australian Laureate Fellowship from the ARC (FL140100052).