A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids

Berit Ebert; Carsten Rautengarten; Heather E McFarlane; Thusitha Rupasinghe; Wei Zeng; Kristina Ford; Henrik V Scheller; Antony Bacic; Ute Roessner; Staffan Persson; Joshua L Heazlewood

Journal article

A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids

Berit Ebert, Carsten Rautengarten, Heather E McFarlane, Thusitha Rupasinghe, Wei Zeng, Kristina Ford, Henrik V Scheller, Antony Bacic, Ute Roessner, Staffan Persson, Joshua L Heazlewood

NATURE PLANTS | NATURE PUBLISHING GROUP | Published : 2018

DOI: 10.1038/s41477-018-0235-5

Abstract

Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis. Most of these reactions occur in the Golgi, requiring cytosolic-derived nucleotide sugars, which need to be actively transferred into the Golgi lumen by nucleotide sugar transporters. We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1). Profiles of N-glycopeptides revealed that plants carrying the ugnt1 loss-of-function allele are virtually devoid of complex..

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University of Melbourne Researchers

Joshua L Heazlewood Author Biosciences

Heather McFarlane Author Biosciences

Carsten Rautengarten Author Biosciences

Staffan Persson Author Biosciences

Ute Roessner Author Biosciences

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Grants

Awarded by Australian Research Council Discovery Project

Awarded by Australian Research Council Future Fellowships

Awarded by Australian Research Council Discovery Early Career Researcher Award

Awarded by Mizutani Foundation for Glycoscience

Awarded by DOE Joint BioEnergy Institute - U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research

Awarded by Australia Research Council Centre of Excellence in Plant Cell Walls

Awarded by NSF-RCN

Funding Acknowledgements

We thank the Biological Optical Microscopy Platform (BOMP) at the University of Melbourne and acknowledge access to the Mass Spectrometry and Proteomics Facility (MSPF) at the Bio21 Institute. Lipid analysis was performed by Metabolomics Australia at the University of Melbourne, a NCRIS initiative under Bioplatforms Australia Pty. The research was supported by an Australian Research Council Discovery Project (DP180102630). B.E. and S.P. are supported by Australian Research Council Future Fellowships (FT160100276 and FT160100218) and H.E.M. is supported by an Australian Research Council Discovery Early Career Researcher Award (DE170100054). This study was also supported by the Mizutani Foundation for Glycoscience (160151) and the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U.S. Department of Energy. W.Z., K.F. and A.B. were supported by the Australia Research Council Centre of Excellence in Plant Cell Walls (CE110001007). The substrates obtained from Carbosource Services (Athens, GA, USA) were supported in part by the NSF-RCN grant no. 0090281.