Human Umbilical Cord Blood Therapy Protects Cerebral White Matter from Systemic LPS Exposure in Preterm Fetal Sheep
Madison CB Paton, Beth J Allison, Jingang Li, Michael C Fahey, Amy E Sutherland, Ilias Nitsos, Robert J Bischof, Justin M Dean, Timothy JM Moss, Graeme R Polglase, Graham Jenkin, Courtney A McDonald, Suzanne L Miller
DEVELOPMENTAL NEUROSCIENCE | KARGER | Published : 2018
BACKGROUND: Infants born preterm following exposure to in utero inflammation/chorioamnionitis are at high risk of brain injury and life-long neurological deficits. In this study, we assessed the efficacy of early intervention umbilical cord blood (UCB) cell therapy in a large animal model of preterm brain inflammation and injury. We hypothesised that UCB treatment would be neuroprotective for the preterm brain following subclinical fetal inflammation. METHODS: Chronically instrumented fetal sheep at 0.65 gestation were administered lipopolysaccharide (LPS, 150 ng, 055:B5) intravenously over 3 consecutive days, followed by 100 million human UCB mononuclear cells 6 h after the final LPS dose. ..View full abstract
This work was made possible with financial support from Inner Wheel Australia, the National Health and Medical Research Council, Australian Research Council, Cerebral Palsy Alliance of Australia, Kahli Sargent Research Studentship, and Australian Government Research Training Program.