Assessing the genetic diversity of Cu resistance in mine tailings through high-throughput recovery of full-length copA genes.
Xiaofang Li, Yong-Guan Zhu, Babak Shaban, Timothy JC Bruxner, Philip L Bond, Longbin Huang
Scientific Reports | Springer Science and Business Media LLC | Published : 2015
Characterizing the genetic diversity of microbial copper (Cu) resistance at the community level remains challenging, mainly due to the polymorphism of the core functional gene copA. In this study, a local BLASTN method using a copA database built in this study was developed to recover full-length putative copA sequences from an assembled tailings metagenome; these sequences were then screened for potentially functioning CopA using conserved metal-binding motifs, inferred by evolutionary trace analysis of CopA sequences from known Cu resistant microorganisms. In total, 99 putative copA sequences were recovered from the tailings metagenome, out of which 70 were found with high potential to be ..View full abstract