Shifts in ovine cardiopulmonary microRNA expression in late gestation and the perinatal period
Ramona H Krauss, Belinda Phipson, Alicia Oshlack, Nikita Prasad-Gupta, Michael M Cheung, Joseph J Smolich, Salvatore Pepe
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2018
BACKGROUND: MicroRNAs (miRNAs) have been identified as important contributors to the regulation of early fetal cardiopulmonary development. However, miRNA expression profiles during late gestation and the early neonatal period are not fully elaborated in large mammals such as sheep (ovis aries). The aim of this study was to sequence miRNA from cardiopulmonary tissues in late gestation and neonate sheep to identify changes in miRNA expression. METHODS: Illumina HiSeq next-generation deep sequencing (NGS) was performed on ovine tissues from the left (LV) and right ventricles (RV), lungs and pulmonary artery (PA) of preterm fetuses (128 days), near-term fetuses (140 days) (term = 148 days) and ..View full abstract
This work was supported in part by the Murdoch Children's Research Institute Clinical Sciences Theme Funding, and the Victorian Government's Operational Infrastructure Support Program. Ramona Krauss was supported by two University of Melbourne International Research Scholarships (MIFRS and MIRS).