Journal article

Optimal protection against Salmonella infection requires noncirculating memory

Joseph M Benoun, Newton G Peres, Nancy Wang, Oanh H Pham, Victoria L Rudisill, Zachary N Fogassy, Paul G Whitney, Daniel Fernandez-Ruiz, Thomas Gebhardt, Pham Quynh-Mai, Lynn Puddington, Sammy Bedoui, Richard A Strugnell, Stephen J McSorley

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2018

Abstract

While CD4 Th1 cells are required for resistance to intramacrophage infections, adoptive transfer of Th1 cells is insufficient to protect against Salmonella infection. Using an epitope-tagged vaccine strain of Salmonella, we found that effective protection correlated with expanded Salmonella-specific memory CD4 T cells in circulation and nonlymphoid tissues. However, naive mice that previously shared a blood supply with vaccinated partners lacked T cell memory with characteristics of tissue residence and did not acquire robust protective immunity. Using a YFP-IFN-γ reporter system, we identified Th1 cells in the liver of immunized mice that displayed markers of tissue residence, including P2X..

View full abstract

Grants

Awarded by NIH T32 training Grant


Awarded by National Institute of Allergy and Infectious Diseases


Awarded by National Health and Medical Research Council (NHMRC) Program


Awarded by NHMRC


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

We thank many past and present members of the S.J.M. laboratory for helpful suggestions as this project developed. Z.N.F. was supported by NIH T32 training Grant AI060555. This study was supported by grants from the National Institute of Allergy and Infectious Diseases (Grant AI056172 to S.J.M. and L.P. and Grants AI103422 and AI139410 to S.J.M.). N.G.P., N.W., and R.A.S. were supported by National Health and Medical Research Council (NHMRC) Program Grant 1092262. S.B. was supported by NHMRC Project Grant 1059937.