Journal article

Transserosal migration of enteric neural stem cells: Developing an avian colon model

Boaz Jurkowicz, Dad Abu-Bonsrah, Dongcheng Zhang, John Hutson, Sebastian King, Don Newgreen

JOURNAL OF PEDIATRIC SURGERY | W B SAUNDERS CO-ELSEVIER INC | Published : 2018

Abstract

BACKGROUND: Stem cell transplantation is a potential therapy for enteric neuropathies, including Hirschsprung disease. Proof-of-principle has been obtained using focal transplants into neonatal mouse colon. The challenge now is to deliver stem cells to a large surface area to reconstruct an enteric nerve plexus. One proposed method is serosal application using a polymer membrane. However, transserosal migration of stem cells has not been demonstrated in mature colon. This study aimed to develop an avian model to demonstrate stem cell migration across the intact serosa of mature colon. METHODS: Hindguts were obtained from E14 quail embryos, transplanted onto E8 chicken chorioallantoic membran..

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Grants

Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

This research was funded, in part, by the National Health and Medical Research Council of Australia (NHMRC 1125805 - Don Newgreen, John Hutson, Sebastian King).