Journal article

Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing

Muhammad Naveed, Samavia Mubeen, SamiUllah Khan, Iftikhar Ahmed, Nauman Khalid, Hafiz Ansar Rasul Suleria, Asghari Bano, Abdul Samad Mumtaz

BRAZILIAN JOURNAL OF MICROBIOLOGY | SPRINGER | Published : 2014

Abstract

In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological..

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University of Melbourne Researchers