Journal article
A Comparative Study of Outer Membrane Proteome between Paired Colistin-Susceptible and Extremely Colistin-Resistant Klebsiella pneumoniae Strains
R Jasim, MA Baker, Y Zhu, M Han, EK Schneider-Futschik, M Hussein, D Hoyer, J Li, T Velkov
ACS Infectious Diseases | AMER CHEMICAL SOC | Published : 2018
Abstract
In the present report we characterized the outer membrane proteome, genomic, and lipid A remodelling changes following the evolution of a colistin-susceptible K. pneumoniae ATCC 13883 strain into an extremely colistin-resistant strain. Lipid A profiling revealed the outer membrane of the colistin-susceptible strain is decorated primarily by hexa- and hepta-acylated lipid A species and a minor tetra-acylated species. In the lipid A profile of the extremely colistin-resistant strain, in addition to the aforementioned lipid A species, the obligatory 4-amino-4-deoxy-l-arabinose modification of the hexa-acylated lipid A was detected. Comparative genomic analysis revealed that the mgrB gene of the..
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Awarded by National Institutes of Health
Funding Acknowledgements
J.L. and T.V. are supported by research grants from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (R01 A1132681). J.L. and T.V. are also supported by the Australian National Health and Medical Research Council (NHMRC) as Senior Research and Career Development Level 2 fellows, respectively. E.K.S.F. is the Young Ambassador to Australia (American Society of Microbiology) and is supported by the NHMRC as an Early Career fellow. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Allergy and Infectious Diseases or the National Institutes of Health.