Journal article

CD8( ) T Cell Activation Leads to Constitutive Formation of Liver Tissue-Resident Memory T Cells that Seed a Large and Flexible Niche in the Liver

Lauren E Holz, Julia E Prier, David Freestone, Thiago M Steiner, Kieran English, Darryl N Johnson, Vanessa Mollard, Anton Cozijnsen, Gayle M Davey, Dale Godfrey, Katsuyuki Yui, Laura K Mackay, Mireille H Lahoud, Irina Caminschi, Geoffrey McFadden, Patrick Bertolino, Daniel Fernandez-Ruiz, William R Heath

CELL REPORTS | CELL PRESS | Published : 2018

Abstract

Liver tissue-resident memory T (Trm) cells migrate throughout the sinusoids and are capable of protecting against malaria sporozoite challenge. To gain an understanding of liver Trm cell development, we examined various conditions for their formation. Although liver Trm cells were found in naive mice, their presence was dictated by antigen specificity and required IL-15. Liver Trm cells also formed after adoptive transfer of in vitro-activated but not naive CD8+ T cells, indicating that activation was essential but that antigen presentation within the liver was not obligatory. These Trm cells patrolled the liver sinusoids with a half-life of 36 days and occupied a large niche that could be a..

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Grants

Awarded by NHMRC program grant


Awarded by NHMRC fellowship


Awarded by NHMRC Senior Principal Research Fellowship


Awarded by NHMRC


Funding Acknowledgements

We thank the BRF facility at the Peter Doherty Institute and the Centenary Institute Animal Facility and Advanced Cytometry Facility for technical support. We also thank Dr. John Cambier for providing the Sting knockout mice. L.E.H., D.N.J., and D.I.G. were supported by the Australian Research Council (Centre for Advanced Molecular Imaging). J.E.P., D.F., and L.K.M. were supported by an NHMRC program grant (APP1129711). D.F.-R., T.M.S., and G.D. were supported by an NHMRC program grant (APP1113293). W.R.H. was supported by an NHMRC fellowship (APP1059861). D.I.G. was supported by an NHMRC Senior Principal Research Fellowship (APP1117766). K.E. and P.B. were supported by NHMRC (project grants APP1108565 and APP1086451). G.I.M. was supported by an NHMRC project grant and an ARC Discovery Project and Laureate Fellowship.