Minimal variation of the plasma lipidome after delayed processing of neonatal cord blood
John M Wentworth, Naiara G Bediaga, Megan AS Penno, Esther Bandala-Sanchez, Komal N Kanojia, Konstantinos A Kouremenos, Jennifer J Couper, Leonard C Harrison
METABOLOMICS | SPRINGER | Published : 2018
BACKGROUND: Cord blood lipids are potential disease biomarkers. We aimed to determine if their concentrations were affected by delayed blood processing. METHOD: Refrigerated cord blood from six healthy newborns was centrifuged every 12 h for 4 days. Plasma lipids were analysed by liquid chromatography/mass spectroscopy. RESULTS: Of 262 lipids identified, only eight varied significantly over time. These comprised three dihexosylceramides, two phosphatidylserines and two phosphatidylethanolamines whose relative concentrations increased and one sphingomyelin that decreased. CONCLUSION: Delay in separation of plasma from refrigerated cord blood has minimal effect overall on the plasma lipidome.
Awarded by National Health and Medical Research Council (NHMRC) of Australia
Awarded by NHMRC
We thank and Ngaire Elwood and Linda Chilcott for assistance with cord blood collection. This research was supported by the Juvenile Diabetes Research Foundation (JDRF) Australia, the Australian Research Council Special Research Initiative in Type 1 Juvenile Diabetes, The Helmsley Charitable Trust, JDRF International, and the National Health and Medical Research Council (NHMRC) of Australia (Program Grants 1037321 and 1054618). LCH is the recipient of a NHMRC Senior Principal Research Fellowship (1080887). The work was made possible through Victorian State Government Operational Infrastructure Support and NHMRC Research Institute Infrastructure Support Scheme.