Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic Domain
Youn-Shen Bee, Yi-Ling Ma, Jinying Chen, Pei-Jhen Tsai, Shwu-Jiuan Sheu, Hsiu-Chen Lin, Hu Huang, Guei-Sheung Liu, Ming-Hong Tai
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | MDPI | Published : 2018
Choroidal neovascularization (CNV) is a key pathological feature of several leading causes of vision loss including neovascular age-related macular degeneration. Here, we show that a calreticulin anti-angiogenic domain (CAD)-like peptide 27, CAD27, inhibited in vitro angiogenic activities, including tube formation, migration of endothelial cells, and vascular sprouting from rat aortic ring explants. In a rat model of laser-induced CNV, we demonstrate that intravitreal injection of CAD27 significantly attenuated the formation of CNV lesions as measured via fundus fluorescein angiography and choroid flat-mounts (19.5% and 22.4% reductions at 10 μg and 20 μg of CAD27 injected, respectively). Si..View full abstract
Awarded by Kaohsiung Veterans General Hospital
Awarded by National Health and Medical Research Council of Australia
We thank Minh Thuan Nguyen Tran for critically reviewing the manuscript. This work was supported by grants from the Kaohsiung Veterans General Hospital (VGHKS 101-056, 103-089), the National Health and Medical Research Council of Australia (#1061912), the Ophthalmic Research Institute of Australia, and the Rebecca L. Cooper Medical Research Foundation.