Development of a rapid colorimetric multiplex PCR-reverse line blot for the detection and typing of 14 Chlamydia trachomatis genovars
Monica Molano, Sepehr N Tabrizi, Samuel Phillips, Jennifer Danielewski, Alyssa Cornall, Servaas A Morre, Suzanne M Garland
JOURNAL OF MEDICAL MICROBIOLOGY | MICROBIOLOGY SOC | Published : 2018
PURPOSE: Chlamydia trachomatis is responsible for trachoma-associated blindness as well as the most common sexually transmitted bacterial infection worldwide, although the genovars for the former are typically A-C, whilst for the latter they are D-K and for the uncommon infection lymphogranuloma venereum they are L1-3. Nucleotide variations within the ompA gene facilitate the identification of C. trachomatis genovars. This study describes a colorimetric multiplex PCR/RLB typing assay (mPCR-RLB) directed to the VD2 region of the ompA gene for general C. trachomatis positivity and the identification of 14 individual C. trachomatis genovars. METHODOLOGY: The assay was validated by analysing 40 ..View full abstract
Awarded by Australian National Health and Medical Research Council
Support was received through an Australian National Health and Medical Research Council Program grant (APP 568971).