Journal article

Development of a rapid colorimetric multiplex PCR-reverse line blot for the detection and typing of 14 Chlamydia trachomatis genovars

Monica Molano, Sepehr N Tabrizi, Samuel Phillips, Jennifer Danielewski, Alyssa Cornall, Servaas A Morre, Suzanne M Garland

JOURNAL OF MEDICAL MICROBIOLOGY | MICROBIOLOGY SOC | Published : 2018

Abstract

PURPOSE: Chlamydia trachomatis is responsible for trachoma-associated blindness as well as the most common sexually transmitted bacterial infection worldwide, although the genovars for the former are typically A-C, whilst for the latter they are D-K and for the uncommon infection lymphogranuloma venereum they are L1-3. Nucleotide variations within the ompA gene facilitate the identification of C. trachomatis genovars. This study describes a colorimetric multiplex PCR/RLB typing assay (mPCR-RLB) directed to the VD2 region of the ompA gene for general C. trachomatis positivity and the identification of 14 individual C. trachomatis genovars. METHODOLOGY: The assay was validated by analysing 40 ..

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Grants

Awarded by Australian National Health and Medical Research Council


Funding Acknowledgements

Support was received through an Australian National Health and Medical Research Council Program grant (APP 568971).