Beyond RGD; nanoclusters of syndecan- and integrin-binding ligands synergistically enhance cell/material interactions
Fatemeh Karimi, Varsha Jagannath Thombare, Craig A Hutton, Andrea J O'Connor, Greg G Qiao, Daniel E Heath
BIOMATERIALS | ELSEVIER SCI LTD | Published : 2018
Biomaterials are a powerful platform for directing cellular behaviour. Herein, we employed a biomimetic strategy to synthesize a low-fouling polymer functionalized with nano-scale clusters of ligands that bind both integrin and syndecan-4 receptors, as both receptor types are critical in focal adhesion signalling and mechanotransduction. Our results demonstrate that the presence of both ligand types synergistically increases the adhesion of human umbilical vein endothelial cells (more than a two fold increase after 4 h) and increases the rate of surface endothelialization compared to surfaces functionalized with only one ligand type. Additionally, we observe that the mixed population of liga..View full abstract
The authors would like to thank the Research Training Program (RTP), the Endeavor IPRS, and the Australian Postgraduate Awards (APA) for providing financial support to this project. We would also like to thank the Particulate Fluids Processing Centre (PFPC), the Materials Characterization and Fabrication Platform (MCFP), and the Biological Optical Microscopy Platform (BOMP).