Journal article

De novo NAD synthesis is required for intracellular replication of Coxiella burnetii, the causative agent of the neglected zoonotic disease Q fever

MA Bitew, CA Khoo, N Neha, DP De Souza, D Tull, NK Wawegama, HJ Newton, FM Sansom

Journal of Biological Chemistry | American Society for Biochemistry and Molecular Biology | Published : 2018

Abstract

© 2018 Bitew et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. Coxiella burnetii is an intracellular Gram-negative bacterium responsible for the important zoonotic disease Q fever. Improved genetic tools and the ability to grow this bacterium in host cell–free media has advanced the study of C. burnetii pathogenesis, but the mechanisms that allow it to survive inside the hostile phagolysosome remain incompletely understood. Previous screening of a transposon mutant library for replication within HeLa cells has suggested that nadB, encoding a putative L-aspartate oxidase required for de novo NAD synthesis, is needed for intracellular..

View full abstract

Grants

Awarded by Australian National Health and Medical Research Council


Funding Acknowledgements

This work was supported in part by Australian National Health and Medical Research Council Grants APP1063646 and APP1120344 (to H. J. N.). The authors declare that they have no conflicts of interest with the contents of this article.