Journal article

Variation in cell-associated unspliced HIV RNA on antiretroviral therapy is associated with the circadian regulator brain-and-muscle-ARNT-like-1

Christina C Chang, Vivek Naranbhai, Jared Stern, Michael Roche, Ashanti Dantanarayana, Ruian Ke, Surekha Tennakoon, Ajantha Solomon, Rebecca Hoh, Wendy Hartogensis, Frederick M Hecht, Ken Sikaris, David J Price, Julian H Elliott, Steven G Deeks, Melissa Churchill, Paul U Cameron, Nicolas Hengartner, Alan S Perelson, Sharon R Lewin

AIDS | LIPPINCOTT WILLIAMS & WILKINS | Published : 2018

Abstract

OBJECTIVE(S): To determine whether variation in cell-associated unspliced (CA-US) HIV RNA in HIV-infected individuals on antiretroviral therapy (ART) has a circadian basis. METHODS: Prospective observational study of HIV-infected individuals on ART. Blood was collected on three occasions and CA-US HIV RNA and mRNA of the circadian-locomotor-output-cycles-kaput (CLOCK)-associated genes quantified by real time PCR. CLOCK-associated proteins were over-expressed in a cell line stably transfected with an HIV long-terminal repeat (LTR) luciferase reporter. RESULTS: Using a mixed effects model, there was a significant increase in log-CA-US RNA at the third visit compared with the first visit (effec..

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Grants

Awarded by National Institutes of Health (NIH) Delaney AIDS Research Enterprise


Awarded by NIH


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Awarded by OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH


Funding Acknowledgements

We acknowledge the patients and staff at Alfred Hospital, UCSF, and The Peter Doherty Institute for Infection and Immunity. This study was supported by the National Institutes of Health (NIH) Delaney AIDS Research Enterprise (DARE U19 AI096109 and UM1 AI126611-01), NIH grants R01-AI028433 and R01-OD011095 (A.S.P.), The American Foundation for AIDS Research (amFAR) Research consortium on HIV eradication (ARCHE) and the National Health and Medical Research Council (NHMRC) of Australia (C.C.C., M.R. and J.H.E. - Early Career Fellowships; and S.R.L. - Practitioner Fellowship).