Journal article

Variability of cortisol assays can confound the diagnosis of adrenal insufficiency in the critically ill population

Jeremy Cohen, Gregory Ward, Johannes Prins, Mark Jones, Bala Venkatesh

INTENSIVE CARE MEDICINE | SPRINGER | Published : 2006

Abstract

OBJECTIVE: To compare the total plasma cortisol values obtained from three widely used immunoassays and a high pressure liquid chromatography (HPLC) technique on samples obtained from patients with sepsis. DESIGN AND SETTING: Observational interventional in the general intensive care unit of a metropolitan hospital PATIENTS AND PARTICIPANTS: Patients admitted to the intensive care unit with a diagnosis of sepsis and fulfilling criteria of systemic inflammatory response syndrome. INTERVENTIONS: Standard short synacthen test performed with 250microg cosyntropin. MEASUREMENTS AND RESULTS: Two of the three immunoassays returned results significantly higher than those obtained by HPLC: Immulite b..

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University of Melbourne Researchers