A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice
Zheng He, Da Zhao, Anna K van Koeverden, Christine T Nguyen, Jeremiah KH Lim, Vickie HY Wong, Algis J Vingrys, Bang Bui
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | JOURNAL OF VISUALIZED EXPERIMENTS | Published : 2018
The circumlimbal suture is a technique for inducing experimental glaucoma in rodents by chronically elevating intraocular pressure (IOP), a well-known risk factor for glaucoma. This protocol demonstrates a step-by-step guide on this technique in Long Evans rats and C57BL/6 mice. Under general anesthesia, a "purse-string" suture is applied on the conjunctiva, around the equator and behind the limbus of the eye. The fellow eye serves as an untreated control. Over the duration of our study, which was a period of 8 weeks for rats and 12 weeks for mice, IOP remained elevated, as measured regularly by rebound tonometry in conscious animals without topical anesthesia. In both species, the sutured e..View full abstract
Awarded by National Health and Medical Research Council of Australia
Awarded by Australian Research Council Future Fellowship
This work is funded by National Health and Medical Research Council of Australia project grant (1046203), Australian Research Council Future Fellowship (FT130100338).