Journal article

Diagnostic application of kidney allograft-derived absolute cell-free DNA levels during transplant dysfunction

John B Whitlam, Ling Ling, Alison Skene, John Kanellis, Francseco L Ierino, Howard R Slater, Damien L Bruno, David A Power

AMERICAN JOURNAL OF TRANSPLANTATION | WILEY | Published : 2019

Abstract

Graft-derived cell-free DNA (donor-derived cell-free DNA) is an emerging marker of kidney allograft injury. Studies examining the clinical validity of this biomarker have previously used the graft fraction, or proportion of total cell-free DNA that is graft-derived. The present study evaluated the diagnostic validity of absolute measurements of graft-derived cell-free DNA, as well as calculated graft fraction, for the diagnosis of graft dysfunction. Plasma graft-derived cell-free DNA, total cell-free DNA, and graft fraction were correlated with biopsy diagnosis as well as individual Banff scores. Sixty-one samples were included in the analysis. For the diagnosis of antibody mediated rejectio..

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