Deep sequencing of primary human lung epithelial cells challenged with H5N1 influenza virus reveals a proviral role for CEACAM1
Siying Ye, Christopher J Cowled, Cheng-Hon Yap, John Stambas
Scientific Reports | NATURE RESEARCH | Published : 2018
Current prophylactic and therapeutic strategies targeting human influenza viruses include vaccines and antivirals. Given variable rates of vaccine efficacy and antiviral resistance, alternative strategies are urgently required to improve disease outcomes. Here we describe the use of HiSeq deep sequencing to analyze host gene expression in primary human alveolar epithelial type II cells infected with highly pathogenic avian influenza H5N1 virus. At 24 hours post-infection, 623 host genes were significantly upregulated, including the cell adhesion molecule CEACAM1. H5N1 virus infection stimulated significantly higher CEACAM1 protein expression when compared to influenza A PR8 (H1N1) virus, sug..View full abstract
This study was supported by funding from The Molecular and Medical Research Strategic Research Centre (MMR SRC), Deakin University and The Geelong Centre for Emerging Infectious Diseases (GCEID) (to S.Y.), and by an Alfred Deakin Postdoctoral Research Fellowship (to S.Y.). The authors wish to thank AAHL CSIRO and the University of Melbourne for providing viruses. We thank Katie-Lee Alexander from Barwon Health Tissue Bank, and Samantha Arandelovic from St John of God Pathology, Geelong, for the coordination of human lung tissue collection and sampling.