Journal article

Development of novel microsatellites for population genetic analysis of Phenacoccus solenopsis Tinsley (Hemipeta: Pseudoccoccidae) based on genomic analysis

Ling Ma, Li-Jun Cao, Ya-Jun Gong, Ary A Hoffmann, Ai-Ping Zeng, Shu-Jun Wei, Zhong-Shi Zhou

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | ELSEVIER SCIENCE BV | Published : 2019

Abstract

The cotton mealybug, Phenacoccus solenopsis Tinsley (Hemipeta: Pseudoccoccidae), is an aggressively invasive pest causing huge economic losses of crops around the world. In this study, we developed genome-wide microsatellites for population genetic analysis of P. solenopsis. We obtained a random genome of P. solenopsis with a size of 267.07 Mb and scaffold N50 of 14.12 Kb. In total 115,639 microsatellites were isolated from the genome, of which those with trinucleotide motifs were the most abundant. Forty-two polymorphic loci were selected for primer validation based on three populations. Allele numbers varied from 2 to 5 with an average value of 2.5 per locus, and allelic richness ranged fr..

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University of Melbourne Researchers

Grants

Awarded by National Key R&D Program of China


Awarded by National Natural Science Foundation of China


Awarded by Natural Science Foundation of Beijing Municipality of China


Awarded by Beijing Key Laboratory of Environmentally Friendly Pest Management on Northern Fruits of China


Funding Acknowledgements

We thank Xu-Bo Wang and Hua-Yan Chen for their helps on the collection of specimens. This research was supported by the National Key R&D Program of China (2016YFC1202100), the National Natural Science Foundation (31472025), the Natural Science Foundation of Beijing Municipality (6162010), The Beijing Key Laboratory of Environmentally Friendly Pest Management on Northern Fruits (BZ0432), all of China.