Journal article

Association of muscle lipidomic profile with high-fat diet-induced insulin resistance across five mouse strains

Magdalene K Montgomery, Simon HJ Brown, Todd W Mitchell, Adelle CF Coster, Gregory J Cooney, Nigel Turner



Different mouse strains exhibit variation in their inherent propensities to develop metabolic disease. We recently showed that C57BL6, 129X1, DBA/2 and FVB/N mice are all susceptible to high-fat diet-induced glucose intolerance, while BALB/c mice are relatively protected, despite changes in many factors linked with insulin resistance. One parameter strongly linked with insulin resistance is ectopic lipid accumulation, especially metabolically active ceramides and diacylglycerols (DAG). This study examined diet-induced changes in the skeletal muscle lipidome across these five mouse strains. High-fat feeding increased total muscle triacylglycerol (TAG) content, with elevations in similar triac..

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University of Melbourne Researchers


Funding Acknowledgements

We thank the Biological Testing Facility at the Garvan Institute (Sydney, Australia) for assistance with animal care. This work was supported by funding from the National Health and Medical Research Council of Australia (NHMRC), the Diabetes Australia Research Trust, the University of Wollongong and the Australian Research Council (ARC). MKM is supported by a NHMRC early career research fellowship.