Association of muscle lipidomic profile with high-fat diet-induced insulin resistance across five mouse strains
Magdalene K Montgomery, Simon HJ Brown, Todd W Mitchell, Adelle CF Coster, Gregory J Cooney, Nigel Turner
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2017
Different mouse strains exhibit variation in their inherent propensities to develop metabolic disease. We recently showed that C57BL6, 129X1, DBA/2 and FVB/N mice are all susceptible to high-fat diet-induced glucose intolerance, while BALB/c mice are relatively protected, despite changes in many factors linked with insulin resistance. One parameter strongly linked with insulin resistance is ectopic lipid accumulation, especially metabolically active ceramides and diacylglycerols (DAG). This study examined diet-induced changes in the skeletal muscle lipidome across these five mouse strains. High-fat feeding increased total muscle triacylglycerol (TAG) content, with elevations in similar triac..View full abstract
We thank the Biological Testing Facility at the Garvan Institute (Sydney, Australia) for assistance with animal care. This work was supported by funding from the National Health and Medical Research Council of Australia (NHMRC), the Diabetes Australia Research Trust, the University of Wollongong and the Australian Research Council (ARC). MKM is supported by a NHMRC early career research fellowship.