Beta-actin does not show the characteristics of a reference protein in human cortex
Georgia M Parkin, Madhara Udawela, Andrew Gibbons, Brian Dean
ELECTROPHORESIS | WILEY | Published : 2019
Levels of a reference protein must be the same as a proportion of total protein in all tissues and, in the study of human diseases, cannot vary with factors such as age, gender or disease pathophysiology. It is increasingly apparent that there may be few, if any, proteins that display the characteristics of a reference protein within the human central nervous system (CNS). To begin to challenge this hypothesis, we used Western blotting to compare variance in levels of the "gold standard" reference protein, β-actin, in Brodmann's area 9 from 194 subjects to variance of total transferred protein measured as intensity of Ponceau S staining. The coefficient of variance of sum intensity measureme..View full abstract
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Awarded by National Health and Medical Research Council (Australia)
This project was supported by the National Health and Medical Research Council (Australia; project grant 566967), Australian Government Research Training Program Scholarship and the Victorian Government's Operational Infrastructure Support Programme. Tissue was sourced from the Victorian Brain Bank, supported by the Mental Health Research Institute, The Alfred, Victorian Forensic Institute of Medicine, The University of Melbourne and funded by Australia's National Health & Medical Research Council, Helen Macpherson Smith Trust, Parkinson's Victoria and Perpetual Philanthropic Services.