Shear Containment of BDNF within Molecular Hydrogels Promotes Human Stem Cell Engraftment and Pos infarc ion Remodeling in Stroke
DR Nisbet, TY Wang, KF Bruggeman, JC Niclis, FA Somaa, V Penna, CPJ Hunt, Y Wang, JA Kauhausen, RJ Williams, LH Thompson, CL Parish
Advanced Biosystems | WILEY-V C H VERLAG GMBH | Published : 2018
Improved control over spatiotemporal delivery of growth factors is needed to enhance tissue repair. Current methods are limited–requiring invasive procedures, poor tissue targeting, and/or limited control over dosage and duration. Incorporation into implantable biomaterials enables stabilized delivery and avoids burst release/fluctuating doses. Here, the physical forces of fibrils formed by self-assembly of epitope-containing peptides are exploited. This biomimetic hydrogel is loaded with neurotrophic factor BDNF via a shear-induced gel–solution transition, unique to noncovalent hydrogels. This results in a biomaterial with three desirable features: a nanofibrillar scaffold, presentation of ..View full abstract
Awarded by NHMRC Dementia Research Leadership Fellowship
D.R.N. and T.Y.W. contributed equally to this work. This research was supported by funding from the National Health and Medical Research Council Australia the Australian Research Council and CASS Foundation, Australia. K.F.B. was supported by a Natural Sciences and Engineering Research Council of Canada Postgraduate Scholarship Doctoral award; D.R.N. was supported by a NHMRC Dementia Research Leadership Fellowship (GNT1135657); C.L.P. was supported by a Senior Medical Research Fellowship provided by the Viertel Charitable Foundation, Australia. The Florey Institute of Neuroscience and Mental Health acknowledges the support from the Victorian Government's Operational Infrastructure Support Grant. Access to the facilities of the Centre for Advanced Microscopy with funding through the Australian Microscopy and Microanalysis Research Facility is gratefully acknowledged. The authors thank Ms Mong Tien for their technical assistance with tissue processing and histochemistry.