Journal article
A surface endogalactanase in Bacteroides thetaiotaomicron confers keystone status for arabinogalactan degradation
A Cartmell, J Muñoz-Muñoz, JA Briggs, DA Ndeh, EC Lowe, A Baslé, N Terrapon, K Stott, T Heunis, J Gray, L Yu, P Dupree, PZ Fernandes, S Shah, SJ Williams, A Labourel, M Trost, B Henrissat, HJ Gilbert
Nature Microbiology | NATURE PUBLISHING GROUP | Published : 2018
Abstract
Glycans are major nutrients for the human gut microbiota (HGM). Arabinogalactan proteins (AGPs) comprise a heterogenous group of plant glycans in which a β1,3-galactan backbone and β1,6-galactan side chains are conserved. Diversity is provided by the variable nature of the sugars that decorate the galactans. The mechanisms by which nutritionally relevant AGPs are degraded in the HGM are poorly understood. Here we explore how the HGM organism Bacteroides thetaiotaomicron metabolizes AGPs. We propose a sequential degradative model in which exo-acting glycoside hydrolase (GH) family 43 β1,3-galactanases release the side chains. These oligosaccharide side chains are depolymerized by the synergis..
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Awarded by European Commission
Funding Acknowledgements
This work was supported in part by an Advanced Grant from the European Research Council (grant no. 322820) awarded to H.J.G. and B.H. supporting D.A.N., A.C., J.M.-M., J.B. and N.T., and a Wellcome Trust Senior Investigator Award to H.J.G. (grant no. WT097907MA) that supported E.C.L. The Biotechnology and Biological Research Council project 'Ricefuel' (grant number BB/K020358/1) awarded to H.J.G. supported A.L. We thank Diamond Light Source for access to beamline I02, I04-1 and I24 (mx1960, mx7854 and mx9948) that contributed to the results presented here.