Journal article
Discovery of a streptococcus pneumoniae serotype 33F capsular polysaccharide locus that lacks wcjE and contains a wcyO pseudogene
S Manna, EM Dunne, BD Ortika, CL Pell, M Kama, FM Russell, T Mungun, E Kim Mulholland, J Hinds, C Satzke
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2018
Abstract
As part of large on-going vaccine impact studies in Fiji and Mongolia, we identified 25/2750 (0.9%) of nasopharyngeal swabs by microarray that were positive for Streptococcus pneumoniae contained pneumococci with a divergent 33F capsular polysaccharide locus (designated ‘33F-1’). We investigated the 33F-1 capsular polysaccharide locus to better understand the genetic variation and its potential impact on serotyping results. Whole genome sequencing was conducted on ten 33F-1 pneumococcal isolates. Initially, sequence reads were used for molecular serotyping by PneumoCaT. Phenotypic typing of 33F-1 isolates was then performed using the Quellung reaction and latex agglutination. Genome assembli..
View full abstractGrants
Awarded by Bill and Melinda Gates Foundation
Funding Acknowledgements
This study was supported by the Bill & Melinda Gates Foundation (OPP1126272 and OPP1084341); Gavi, the Vaccine Alliance; and the Department of Foreign Affairs and Trade of the Australian Government and Fiji Health Sector Support Program (FHSSP). CS holds a NHMRC Career Development Fellowship and a veski Inspiring Women Fellowship. SM received a Robert Austrian Research Award in Pneumococcal Vaccinology funded by Pfizer. This work was also supported by the Victorian Government's Operational Infrastructure Support Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.