Journal article

Secreted and intracellular cytokines are complementary measures for human monocytes treated with Toll-like receptor agonists

Yang Song, Julie Nigro, Lijia Yu, Mario Congiu, Narelle Skinner, Alexander Thompson, Kumar Visvanathan

Journal of Immunological Methods | ELSEVIER | Published : 2019

Abstract

Cytokine production by human peripheral blood mononuclear cells including monocytes, is frequently assessed by measuring secreted cytokines using enzyme linked immunosorbent assay (ELISA), whereby the total concentration of one cytokine of interest is obtained without information regarding the cell type responsible for making the cytokine. Cytokines can be retained inside the cell using protein transport inhibitors. Subsequent analysis by flow cytometry not only identifies the cell type producing the cytokine but can semi-quantitate the amount of cytokine produced by measuring the geometric mean fluorescence intensity (gMFI) and is amenable to analyzing more than one protein associated with ..

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Grants

Awarded by Australian National Health and Medical Research Council


Funding Acknowledgements

This work was supported by a project grant to KV from the Australian National Health and Medical Research Council (ID: 1066536). YS was supported by the China Scholarship Council.