Characterization of Staphylococcal Cassette Chromosome mec Elements from Methicillin-Resistant Staphylococcus pseudintermedius Infections in Australian Animals
Kate A Worthing, Sybille Schwendener, Vincent Perreten, Sugiyono Saputra, Geoffrey W Coombs, Stanley Pang, Mark R Davies, Sam Abraham, Darren J Trott, Jacqueline M Norris
MSPHERE | AMER SOC MICROBIOLOGY | Published : 2018
We examined the oxacillin resistance phenotype and genomic structure of staphylococcal cassette chromosome mec (SCCmec) elements from 77 veterinary methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates. Isolates were characterized by oxacillin broth microdilution, whole-genome sequencing, and bioformatics analysis. Five previously described SCCmec elements, and a sixth novel element, were identified: SCCmec III (also known as II-III), ΨSCCmec 57395, and SCCmec NA45 (a SCCmec VII variant), all previously described in MRSP, and SCCmec IVg and SCCmec VT, previously described in both methicillin-resistant Staphylococcus aureus (MRSA) and MRSP. The sixth element was novel and foun..View full abstract
Awarded by Australian Research Council-Linkage Grant
The collection of the 77 MRSP isolates was supported by Zoetis Pty Ltd. and the Australian Research Council-Linkage Grant (grant number LP130100736). Sequencing of Psi SCCmec<INF>KW21</INF> was supported by internal funds of the Institute of Veterinary Bacteriology of the University of Bern, Bern, Switzerland.