Journal article

Cardiac troponins may be irreversibly modified by glycation: novel potential mechanisms of cardiac performance modulation

Johannes V Janssens, Brendan Ma, Margaret A Brimble, Jennifer E Van Eyk, Lea MD Delbridge, Kimberley M Mellor

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2018

Abstract

Dynamic movements of the cardiac troponin complex are an important component of the cardiac cycle. Whether cardiac troponins are subjected to irreversible advanced glycation end-product (AGE) modification is unknown. This study interrogated human and rat cardiac troponin-C, troponin-I and troponin-T to identify endogenous AGE modifications using mass spectrometry (LC-MS/MS). AGE modifications were detected on two amino acid residues of human troponin-C (Lys6, Lys39), thirteen troponin-I residues (Lys36, Lys50, Lys58, Arg79, Lys117, Lys120, Lys131, Arg148, Arg162, Lys164, Lys183, Lys193, Arg204), and three troponin-T residues (Lys107, Lys125, Lys227). AGE modifications of three corresponding ..

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