Journal article

RNA Catabolites Contribute to the Nitrogen Pool and Support Growth Recovery of Wheat

Vanessa Jane Melino, Alberto Casartelli, Jessey George, Thusitha Rupasinghe, Ute Roessner, Mamoru Okamoto, Sigrid Heuer

FRONTIERS IN PLANT SCIENCE | FRONTIERS MEDIA SA | Published : 2018

Abstract

Turn-over of RNA and catabolism of nucleotides releases one to four ammonia molecules; the released nutrients being reassimilated into primary metabolism. Preliminary evidence indicates that monocots store high levels of free nucleotides and nucleosides but their potential as a source of internal organic nitrogen for use and remobilization is uncharted. Early tillering wheat plants were therefore starved of N over a 5-day time-course with examination of nucleic acid yields in whole shoots, young and old leaves and roots. Nucleic acids constituted ∼4% of the total N pool of N starved wheat plants, which was comparable with the N available from nitrate (NO3 -) and greater than that available f..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council


Funding Acknowledgements

This work was supported by an Australian Research Council linkage grant LP1400100239 in partnership with and additional funding from DuPont-Pioneer (United States). LC-MS analysis was carried out at Metabolomics Australia, School of BioSciences, The University of Melbourne, which is supported by funds from the Australian Government's National Collaborative Research Infrastructure Scheme (NCRIS) administered through Bioplatforms Australia (BPA) Ltd. Rothamsted Research is supported by the Biotechnology and Biological Sciences Research Council (BBSRC, United Kingdom), a part of Research Innovation UK.