Journal article

Defining the in vivo characteristics of acute myeloid leukemia cells behavior by intravital imaging

Delfim Duarte, Saoirse Amarteifio, Heather Ang, Isabella Y Kong, Nicola Ruivo, Gunnar Pruessner, Edwin D Hawkins, Cristina Lo Celso

IMMUNOLOGY AND CELL BIOLOGY | WILEY | Published : 2019

Abstract

The majority of acute myeloid leukemia (AML) patients have a poor response to conventional chemotherapy. The survival of chemoresistant cells is thought to depend on leukemia-bone marrow (BM) microenvironment interactions, which are not well understood. The CXCL12/CXCR4 axis has been proposed to support AML growth but was not studied at the single AML cell level. We recently showed that T-cell acute lymphoblastic leukemia (T-ALL) cells are highly motile in the BM; however, the characteristics of AML cell migration within the BM remain undefined. Here, we characterize the in vivo migratory behavior of AML cells and their response to chemotherapy and CXCR4 antagonism, using high-resolution 2-p..

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University of Melbourne Researchers

Grants

Awarded by GABBA PhD program (FCT fellowship)


Awarded by Bloodwise


Awarded by HFSP


Awarded by CRUK


Awarded by BBSRC


Awarded by ERC


Awarded by KKLF


Funding Acknowledgements

DD was funded by the GABBA PhD program (FCT fellowship SFRH/BD/52195/2013) and by the EHA-ASH TRTH program; CLC by Bloodwise (12033 and 15031), HFSP (RGP0051/2011), CRUK (C36195/A1183), BBSRC (BB/I004033/1) KKLF (KKL460) and ERC (337066); EH by the European Hematology Association (EHA), Bloodwise (12033) and NHMRC. We thank D Keller, S Rothery (Imperial College FILM facility), and N Sergent (Carl Zeiss) for support with microscopy; S Piperelis, E Ibarguen, W Steel, H Goyal, and C Godfrey (Imperial College CBS facility) for logistical help; J Srivastava, C Simpson, and J Rowley for support from the flow cytometry facility; and T Luis (Imperial College London) for the critical reading of the manuscript.