Journal article

Defining the in vivo characteristics of acute myeloid leukemia cells behavior by intravital imaging

Delfim Duarte, Saoirse Amarteifio, Heather Ang, Isabella Y Kong, Nicola Ruivo, Gunnar Pruessner, Edwin D Hawkins, Cristina Lo Celso



The majority of acute myeloid leukemia (AML) patients have a poor response to conventional chemotherapy. The survival of chemoresistant cells is thought to depend on leukemia-bone marrow (BM) microenvironment interactions, which are not well understood. The CXCL12/CXCR4 axis has been proposed to support AML growth but was not studied at the single AML cell level. We recently showed that T-cell acute lymphoblastic leukemia (T-ALL) cells are highly motile in the BM; however, the characteristics of AML cell migration within the BM remain undefined. Here, we characterize the in vivo migratory behavior of AML cells and their response to chemotherapy and CXCR4 antagonism, using high-resolution 2-p..

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University of Melbourne Researchers


Awarded by GABBA PhD program (FCT fellowship)

Awarded by Bloodwise

Awarded by HFSP

Awarded by CRUK

Awarded by BBSRC

Awarded by ERC

Awarded by KKLF

Funding Acknowledgements

DD was funded by the GABBA PhD program (FCT fellowship SFRH/BD/52195/2013) and by the EHA-ASH TRTH program; CLC by Bloodwise (12033 and 15031), HFSP (RGP0051/2011), CRUK (C36195/A1183), BBSRC (BB/I004033/1) KKLF (KKL460) and ERC (337066); EH by the European Hematology Association (EHA), Bloodwise (12033) and NHMRC. We thank D Keller, S Rothery (Imperial College FILM facility), and N Sergent (Carl Zeiss) for support with microscopy; S Piperelis, E Ibarguen, W Steel, H Goyal, and C Godfrey (Imperial College CBS facility) for logistical help; J Srivastava, C Simpson, and J Rowley for support from the flow cytometry facility; and T Luis (Imperial College London) for the critical reading of the manuscript.