Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells
Mohd Hafiz Arzmi, Nicola Cirillo, Jason C Lenzo, Deanne V Catmull, Neil O'Brien-Simpson, Eric C Reynolds, Stuart Dashper, Michael McCullough
CARCINOGENESIS | OXFORD UNIV PRESS | Published : 2019
Microbial infection has been shown to involve in oral carcinogenesis; however, the underlying mechanisms remain poorly understood. The present study aimed to characterize the growth of oral microorganisms as both monospecies and polymicrobial biofilms and determine the effects of their products on oral keratinocytes. Candida albicans (ALC3), Actinomyces naeslundii (AN) and Streptococcus mutans (SM) biofilms or a combination of these (TRI) were grown in flow-cell system for 24 h. The biofilms were subjected to fluorescent in situ hybridization using species-specific probes and analysed using confocal laser scanning microscopy. The effluent derived from each biofilm was collected and incubated..View full abstract
Awarded by International Islamic University Malaysia, Kuantan, Pahang, Malaysia
The project was funded by OH-CRC, The University of Melbourne and the International Islamic University Malaysia, Kuantan, Pahang, Malaysia (RIGS17-083-0658).