Sample-to-answer on molecular diagnosis of bacterial infection using integrated lab--on--a--disc.
JFC Loo, HC Kwok, CCH Leung, SY Wu, ILG Law, YK Cheung, YY Cheung, ML Chin, P Kwan, M Hui, SK Kong, HP Ho
Biosensors and Bioelectronics | Published : 2017
Sepsis by bacterial infection causes high mortality in patients in intensive care unit (ICU). Rapid identification of bacterial infection is essential to ensure early appropriate administration of antibiotics to save lives of patients, yet the present benchtop molecular diagnosis is time-consuming and labor-intensive, which limits the treatment efficiency especially when the number of samples to be tested is extensive. Therefore, we hereby report a microfluidic platform lab-on-a-disc (LOAD) to provide a sample-to-answer solution. Our LOAD customized design of microfluidic channels allows automation to mimic sequential analytical steps in benchtop environment. It relies on a simple but contro..View full abstract