Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis
Miguela Martin, David Perez-Guaita, Dean W Andrew, Jack S Richards, Bayden R Wood, Philip Heraud
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | JOURNAL OF VISUALIZED EXPERIMENTS | Published : 2018
We demonstrate a method of quantification and detection of parasites in aqueous red blood cells (RBCs) by using a simple benchtop Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectrometer in conjunction with Multivariate Data Analysis (MVDA). 3D7 P. falciparum were cultured to 10% parasitemia ring stage parasites and used to spike fresh donor isolated RBCs to create a dilution series between 0-1%. 10 µL of each sample were placed onto the center of the ATR diamond window to acquire the spectrum. The sample data was treated to improve the signal to noise ratio and to remove the contribution of water, and then the second derivative was applied to resolve spectral features...View full abstract
Awarded by Australian Research Council
Funding to the authors was provided by the Australian Research Council (Future Fellowship FT120100926 to BRW), National Health and Medical Research Council of Australia (Program grant and Senior Research Fellowship to JGB; Early Career Fellowships JSR; Infrastructure for Research Institutes Support Scheme Grant to the Burnet Institute), and the Victorian State Government Operational Infrastructure Support Grant to the Burnet Institute. We acknowledge Mr. Finlay Shanks for instrumental support.