Journal article
Photosensitised Multiheme Cytochromes as Light-Driven Molecular Wires and Resistors
JH van Wonderen, D Li, SEH Piper, CY Lau, LP Jenner, CR Hall, TA Clarke, NJ Watmough, JN Butt
Chembiochem | WILEY-V C H VERLAG GMBH | Published : 2018
Abstract
Multiheme cytochromes possess closely packed redox-active hemes arranged as chains spanning the tertiary structure. Here we describe five variants of a representative multiheme cytochrome engineered as biohybrid phototransducers for converting light into electricity. Each variant possesses a single Cys sulfhydryl group near a terminus of the heme chain, and this was efficiently labelled with a RuII(2,2′-bipyridine)3 photosensitiser. When irradiated in the presence of a sacrificial electron donor (SED) the proteins exhibited different types of behaviour. Certain proteins were rapidly and fully reduced. Other proteins were rapidly semi-reduced but resisted complete photoreduction. These findin..
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Awarded by Collaborative Innovation Center of Suzhou Nano Science and Technology
Funding Acknowledgements
We would like to thank Jochen Blumberger, Antoine Carof, Xiuyun Jiang, Steve Meech and John Fielden for insightful discussions. We are grateful for assistance from Simone Payne for protein purification, Jason Crack for LC-MS, Myles Cheesman and Justin Bradley for MCD, Marcus Edwards for help with molecular biology, Jack McLeish for initial photoreduction studies and the EPSRC UK National Mass Spectrometry Facility at Swansea University for characterisation of RuMeBr. Funding was from the UK Engineering and Physical Sciences Research Council (EP/M001989/1, EP/N033647/1) and the Biotechnology and Biological Sciences Research Council (BB/L022176/1, BB/K009885/1 and Doctoral Training Partnership PhD studentship to SEHP). D.L. is grateful to the Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO-CIC) and the Chinese Collaborative Academic Training Program for Post-doctoral Fellows. J.N.B. acknowledges a Royal Society Leverhulme Trust Senior Research Fellowship.