Journal article

Effect of peripheral blood mononuclear cell cryopreservation on innate and adaptive immune responses

Jeremy Anderson, Zheng Quan Toh, Andrea Reitsma, Anh Ha Do Lien, Jordan Nathanielsz, Paul Licciard

Journal of Immunological Methods | ELSEVIER | Published : 2019

Abstract

Cryopreservation of blood-derived immune cells is commonly used in clinical trials to examine immunological responses. However, studies elucidating the effects of cryopreservation on peripheral blood mononuclear cell (PBMC) responses have shown inconsistent results making it difficult to draw meaningful conclusions. Therefore we sought to address this issue by comparing key innate and adaptive immune parameters between freshly-isolated and cryopreserved PBMCs from healthy adults. We examined the effect of cryopreservation on the expression of key markers on innate and adaptive immune cell populations (i.e. CD4+ and CD8+ [T cells], CD14+ [monocytes], CD19+ [B cells], CD56+ [NK cells] or CD19 ..

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Grants

Funding Acknowledgements

We thank all the study participants for their involvement in this study. P.V.L conceived and designed the experiments; J.A, Z.Q.T and A.R performed the experiments; J.A, Z.Q.T, P.V.L and A.R analysed the data; J.A, P.V.L, Z.Q.T, L.A.H.D and J.N wrote the paper. P.V.L was supported by a Career Development Fellowship from the Australian National Health and Medical Research Council. We also acknowledge the Victorian Government's Operational Infrastructure Support Program.