Journal article

A Liposomal Platform for Sensing of Extracellular Analytes Near Cells

Xiaozhou Zhang, Sabrina Heng, Jinxin Pei, Jacqueline R Morey, Christopher A McDevitt, Andrew D Abell

BIOSENSORS-BASEL | MDPI | Published : 2018


Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca2+, GSH, and Zn2+, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca2+ (0.25 mM⁻1 mM), Zn2+ (6.25 µM⁻50 µM), and GSH (0.25 mM⁻1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn2+ (1 mM), Ca2+ (1 mM), or GSH (1 mM) near..

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Awarded by National Health and Medical Research Council

Awarded by Australian Research Council (ARC)

Awarded by ARC

Funding Acknowledgements

National Health and Medical Research Council (Project Grants 1080784 and 1122582); Australian Research Council (ARC) DP170102102/FT170100006; ARC CE140100 003; the University of Adelaide Barbara Kidman Fellowship; the OptoFab node of the Australian National Fabrication Facility (ANFF).