Journal article

A Liposomal Platform for Sensing of Extracellular Analytes Near Cells

Xiaozhou Zhang, Sabrina Heng, Jinxin Pei, Jacqueline R Morey, Christopher A McDevitt, Andrew D Abell

BIOSENSORS-BASEL | MDPI | Published : 2018

Abstract

Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca2+, GSH, and Zn2+, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca2+ (0.25 mM⁻1 mM), Zn2+ (6.25 µM⁻50 µM), and GSH (0.25 mM⁻1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn2+ (1 mM), Ca2+ (1 mM), or GSH (1 mM) near..

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Grants

Awarded by National Health and Medical Research Council


Awarded by Australian Research Council (ARC)


Awarded by ARC


Funding Acknowledgements

National Health and Medical Research Council (Project Grants 1080784 and 1122582); Australian Research Council (ARC) DP170102102/FT170100006; ARC CE140100 003; the University of Adelaide Barbara Kidman Fellowship; the OptoFab node of the Australian National Fabrication Facility (ANFF).